Sentence examples for protocol of reading from inspiring English sources

To overcome this PE read imbalance in targeted sequencing, we modified the standard PE sequencing protocol of reading the forward and reverse strand of a DNA template during one PE read.

We found that for the reads obtained using a standard miRNA-seq protocol, the number of reads aligned to tDRs (78.81 % of reads) vastly outnumbered those aligning to miRNAs (4.43 % of reads) (Fig.  1a).

Our porcine specific GR primers and protocol minimize the number of reads of globin transcripts in whole blood samples and provides increased coverage as well as accuracy and reproducibility of transcriptome analysis.

Most SGS platforms propose paired-end sequencing protocols which provide pairs of reads.

Future optimization of the WMD-MSG protocol may allow improved normalization of read counts among samples, although the current performance of the protocol was sufficient for our study of 384 individuals.

5'-ESS sequences were rather rare in the Illumina GA data (0.16%), and were absent in the Illumina cloning data with the two version protocols due to limited counts of reads, but 5'-ESS accounted for 10%and6.3%3% for SOLiD cloning data of Hu sheep and Dorper sheep, respectively.

Pre-processing of reads was done with standard protocols (see Materials and Methods).

bMRI requires standardisation of patient-management, protocols, and reading set-up.

A pathologist expert analyzed all samples after standardization of the criteria using consensus protocol reading [ 25].

Some protocols use a PCR step, which may lead to the spurious replication of reads.

Using the final protocol, we reproducibly obtained just under 50% uniquely mapping reads (out of the total number of reads that passed the Illumina pipeline quality filter).