Sentence examples for protocol of mixing from inspiring English sources

The protocol of mixing was varied, and, its effect on the mechanical properties was investigated.

The assay protocol consisted of repeated cycles of mixing (3 min), incubation (2 min) and then measurement (3 min) periods.

A similar protocol was used for the PV6C sediment, except that the samples were retrieved after 12, 24, and 48 h of mixing.

Direct effects of mixing.

Beware of mixing.

The steps were performed as follows: a 10 µl aliquot of the P-labeled product from the final extension reaction of any of the detection protocols, was mixed with 4 µl of sequencing stop solution (11) and loaded onto a 6% PAGE gel (0.33 mm thick) under denaturing conditions (11).

Isolation of total RNA including small RNA was performed with miRNeasy kit (Qiagen) according to the manufacturer's protocol with the exceptions of mixing 2 mL of Qiazol reagent with 0.4 mL serum, loading the entire aqueous phase onto a single column from the MinElute Cleanup Kit (Qiagen), and eluting the RNA in 20 μL of RNase-free water.

The sensitivity of the protocol was determined after mixing a Jurkat T-cell clone in a background of peripheral blood CD4+ T-cells in a range of dilutions.

With this protocol a mix of oocytes is obtained, some with and some without Mps1 or Polo localizing to filaments, due to some females being exposed to CO2 for longer periods of time.

The single-cell suspension step integral to this protocol is ideal for mixing experiments, and will also facilitate genetic manipulation of the hESC.

cDNA was then synthesized by reverse transcription of total RNA using Supercript™III reverse transcriptase included in the RT enzyme mix and RT reaction mix according to the protocol of the manufacturer (Invitrogen).