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We calculated the relative efficiency of labeling measuring the base/dye ratio as described by Invitrogen's protocol of labeling with amine-reactive reagent.
All the micro-array experiments were performed in our laboratory with a normalized protocol of labeling, hybridization, data normalization and statistical analysis ensuring a perfect homogeneity of the data.
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Based on preliminary data showing that LNA-based capture probes were highly sensitive for miRNA (to at least 50 attomoles), we developed a 90-min, straightforward protocol for labeling small amounts of RNA (tested down to 1 μg), without the need for miRNA enrichment.
This particular protocol was chosen for ease of labeling surface proteins without removing drug treatments; however, similar results were obtained if cells were detached and suspended, followed by sequential addition of dyes prior to analysis without any washing steps.
Our quantification protocol of cells labeled with the DAB-peroxidase method, e.g. BrdU-positive cells in this study, has been described elsewhere (Kempermann et al. 2003) and has been used in numerous studies thereafter.
For a detailed description of our protocol for labeling of ODNs with P, see [ 79].
Using standard hybridization protocols, 5.5 μg of labeled cRNA was hybridized to the Affymetrix Toxoplasma GeneChip [ 26] and imaged with an Affymetrix GeneChip Scanner (Affymetrix).
The protocol of iTRAQ labelling was followed the company manual.
However, patients who were taking aspirin and unable to cease were eligible to be enrolled into the expanded protocol of open-label aspirin.
Following the Arabidopsis protocols [ 31], 40 μg of labeled product was obtained from 300 ng of rice gDNA.
The first protocol yielded a high concentration ratio of labeled target to interactor, while the second yielded a low ratio of target to interactor.
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