All procedures from recruitment and intervention to follow-up were strictly monitored [ 19] by completion of forms, and the occupational therapists closely followed the protocol of clinical instructions for performing the intervention programme during the study period.
The soluble 7ZFPs were purified from the sonicated supernatants through incubation with Ni-NTA-Sepharose beads (GE Healthcare, German) for 1 hr, and eluted from the beads with 500 mmol/L of imidazole, according to the protocol of the instruction manual.
In anticipation of the execution, and others to come, the bureau has compiled an "Execution Protocol," 56 pages of instructions to be carried out by the staff of the federal penitentiary in Terre Haute, Ind., which has the only federal death chamber in the country.
A protocol, a set of instructions, and definitions does not, however, take the place of expert judgment.
The procedures of RT-PCR analysis were according to the protocol of manufacturer's instructions (Takara, Dalian, China).
The procedures of total RNA extration were according to Trizol reagent protocol of manufacturer's instructions (invitrogen, Life Technologies, Ca, USA).
Approximately 90percentt of protocol instructions were followed in both groups, with a 1percentt rate of crossover from CVC- to PAC-guided therapy.
The EMSA assay was carried out according to the protocol of the Kit instruction as described previously [ 22].
To the best of the knowledge of the authors, this is the first published protocol providing a generally applicable set of instructions for the comparison of RNA-seq data between different species.
Culture supernatants were harvested and detected for IFN-γ at 96 h, IL-2 at 24 h, IL-4 at 24 h and IL-10 at 72 h according to a protocol of the manufacture's instruction.
