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A complex protocol of data and specimen collection was applied, including an interview, pelvic exam for sexually active women, blood for serology and cell-mediated immunity, cervical secretions for local immunity and cells for HPV, Chlamydia trachomatis and gonorrhea testing.
The huge number of statistical tests performed requires a severe statistical cut-off, or a protocol of data filtering, to be able to select only the most promising results.
In summary, we demonstrated nanoscale far-field fluorescence imaging based on reversible photoswitching and detecting the photon bursts of individual molecules with recording times of 2 2.5 min. These advancements are due to an asynchronous laissez-faire protocol of data acquisition that just matches the camera acquisition time to the duration of the photon bursts.
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This disparity calls for the creation of protocols of data collection, in order to homogenize existing dataset and increase the possibility of comparisons between the different data collections.
The Pipeline environment allows the development of new data input and output parsers for streamlining the protocols of data management and utilization within the Pipeline.
The pilot study also served to train evaluators and standardize planned protocols of data collection.
HW: design of protocol, evaluation of data, draft of the manuscript.
MB was involved with the engineering design, OCT protocol, evaluation of data, and writing of manuscript.
BM, TS and EV significantly contributed to devising the protocol, analysis of data and revision of the manuscript.
BKP: chief investigator of MOSTI grant, development of research protocol, supervision of data collection, interpretation of results, and editing of manuscript.
Specific areas to be reviewed included the integrity of the study protocol, testing of data collection forma and acceptability of the intervention.
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