Robust protocols for the assembly of nucleosomes and chromatin templates have been developed for structural, biochemical, and biophysical studies.
For the assembly of various B6Fab derivatives, a protocol comprising of in vitro denaturation of Fd/Fdenv and LC, and their subsequent renaturation and association was optimised.
Hmwk is important for the assembly of plasma kallikrein.
Here we examined four different methodologies for the assembly of bacterial genomes: short read only assembly, hybrid scaffolding, hybrid assembly, and PacBio-only assembly.
For the assembly of the sequence reads we applied GS De Novo Assembler Software (2.3) using transcriptome assembly default settings.
Adapting molecular biology to materials science we developed peptide-based protocols for the assembly and formation of hybrid materials and systems.
Further, we report to our knowledge, the simplest and fastest protocol for the recovery and assembly of complete mitochondrial genomes using the MiSeq benchtop sequencer.
To address this challenge we have devised a simple, reliable, and universal protocol for the RBS assembly that can potentially also be applied to other small parts assembly.
This property has been used to develop protocols for efficient assembly of multiple DNA fragments in a single ligation reaction, for mutagenesis, and for cloning of fragments with seamless junctions [4], [5], [6], [7], [8] (for reviews [9], [10], [11]).
