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A protocol for the application of the BSB technology and its associated maintenance strategy is defined.
Here we provide a step-by-step protocol for the application of synthetic theophylline-dependent riboswitches for conditional gene expression in Streptomyces coelicolor.
To take advantage of preserved patient tissue we investigated the sample quality requirements of formaldehyde-fixed and paraffin-embedded (FFPE) tissues and established a protocol for the application of massively parallel short-read sequencing approaches.
This publication brings a new and more efficient protocol for the application of FISH technique for the first time in meiotic pachytene chromosomes of coffee.This new protocol involves some procedures for obtain suitable pachytene chromosomes that allows the making of a higher-resolution cytogenetic mapping on coffee chromosomes than that mapping on mitotic chromosomes.
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In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.
Detailed protocols for the application of real-time reverse transcription polymerase chain reaction and immunohistochemistry in target gene expression analysis are described.
These encompass protocols for the application of homemade microarrays and custom‐designed commercial inkjet microarrays and are tailored for the large‐scale analysis of processing of mRNA, including alternative splicing, as well as for the analysis of processing and modification of noncoding RNA.
To gain knowledge on the existence or not of protocols for the application of coercive measures in Spanish general hospitals, and to gain knowledge of the measures that are actually applied in practice and to analyze ethical, medico-legal and legal problems generated, especially in relation to the rights of the ill citizen.
When examining the effectiveness of behavioural treatment for chronic pain another difficulty is that no standard protocol exists for the application of these treatments.
Additionally, our protocol allows for the application of ice packs to the groin and axilla, and a cooling blanket (Maxi-Therm® Lite Blankets, Cincinnati Sub-Zero, Cincinatti, OH) directly to the patients skin during the induction and maintenance phases of hypothermia.
The interviews were conducted individually via Skype, following protocol and script, designed for the application of the instrument, where the interviewer created a bond of trust with the interviewees and thus achieved an in-depth interview.
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CEO of Professional Science Editing for Scientists @ prosciediting.com