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The protocol for structure standardisation.
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As mentioned earlier, we use a 3-fold protocol for inferring structure using BLASTp, I-TASSER, and RosettaDock.
Three-dimensional structures of SLVI with bound Mg(H2O) n 2+ complexes were calculated by restraining molecular dynamics and simulated annealing with X-PLOR-NIH version 2.1.9 by adapting the two-stage protocol previously used for structure determination of free SLVI.
In essence the ITU wants to regulate the way the net operates, establishing protocols for the structure of service delivery and appropriate content.
Here, we present an in silico protocol for predicting peptide MHC structure.
Using a combination of experimental and computational techniques, the paper provides a general protocol for studying the structure in solution of molecular systems characterized by the existence of conformational metastable states.
Here we focus primarily on developing the protocol for mesoscopic scale structure analysis.
Further, given the accuracy of the newly available mutate-and-map protocol for inferring secondary structure, we sought to only use MOHCA-seq to determine tertiary proximities for nucleotide pairs that are far in secondary structure.
For FG-pulldowns, Ran-binding assays and protein translocation assays, the Mt proteins were purified using the same protocol that was used for structure determination, except for omitting the first HiTrapS FF column step.
Dimaio et al. recently described a Rosetta-based rebuilding-and-refinement protocol for fitting protein structures into density maps.
However, the availability of sufficient purification protocols for these structures is rather limited and restricted to density gradient centrifugation.
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