Sentence examples for protocol for purification from inspiring English sources

This review outlines a detailed protocol for purification of native Cre and describes straightforward assays that can be used to test for recombination activity in vitro.

Here, we present a simple protocol for purification of functionally active human DHDDS under non-denaturating conditions using a codon-optimized construct.

13568_2014_83_MOESM1_ESM.docx Additional file 1: Protocol for purification of PCT.

A protocol for purification of SELP-109-A will be further developed, possibly involving the use of chaotropic agents in the lysis buffer in order to inhibit hydrogen bonding.

Since the incubation times are much shorter using the protocol for purification via functionalized magnetic beads, it was suggested that the hydantoinase was maybe exposed to proteolytic digestion during the incubation of the crude cell extract with the Ni Sepharose beads in spite of the added protease inhibitor.

Protocol for purification of PP2A proteins: trimeric PP2A1 purification from pig brain was adapted from methods previously described [43].

We modified earlier protocols for purification of DmmtSSB [8] and HsmtSSB [15] in order to obtain highly pure proteins (Fig. 2).

We have established protocols for purification of these two CCN, and biochemically and biologically characterised the recombinant proteins by mass spectrometry, tryptic in-gel-digests, and proliferation and gene reporter assays in EAhy 926 cells.

Standard molecular biological protocols for purification of DNA from agarose gels, DNA restriction and ligation, transformation of E. coli Top10 (Invitrogen) to ampicillin resistance, and plasmid mini-DNA preparations were employed.

In the present study we set up a size-exclusion chromatography-based, scalable purification protocol for the purification of a VLP-based influenza A vaccine produced in Escherichia coli.

The final protocol used for purification of the spinae consists of washing and resuspending the harvested cells with deionized water, then mechanically shearing to detach the spinae and collecting them by centrifugation (see Methods for details).