Thus, albeit the need for cloning, a medium-throughput protocol for gain-of-function screen of selected microRNAs could be established, which identified positive effects for miR-17 on CHO growth performance (Jadhav et al., 2012).
In a letter the month before, the governor accused Mr. French of several transgressions, including disclosing to a journalist details of where the KIC-1 files were kept and the protocols for gaining access to them.
Also, in the ecotoxicology science community of chemicals, there is an ongoing, lively discussion on these issues spanning now at least four decades and the field is constantly striving for improved protocols to gain more relevance for field situations.
Council attendance was determined by locality, sex, age, and the specific question at hand; each council had its own protocol and devices for gaining consensus, which was the primary mode of decision making.
There are different protocols for students to gain lab access than there are for post-docs.
By introduction of the protocol of Wiley and colleagues [11] and omission of the gel purification initially included in the sequencing protocol for highly pathogenic influenza A H5N1 [9], we gain the possibility to fully automate sample preparation.
Having a better understanding of both standards could help in designing and developing efficient network architecture and protocols for further performance gains.
However, when we employed a previously described protocol for planktonic stages, no reproducible down-regulation of target genes was gained.
We applied the IM protocol for the development of an intervention aiming at the prevention of excessive weight gain among Dutch adolescents.
We followed the manufacturer's protocol for creating genomic DNA libraries with a few modifications based on experience gained from ChIP-Sequencing [ 6].
Information gained from this study will help in guiding development of optimal protocol for cryopreservation of fish oocytes.
