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Normal mouse IgG was coupled to tosylactivated Dynabeads using the protocol for cell separation applications as recommended by the manufacturer.
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For cell separation AutoMACS Pro (Miltenyi Biotech) was used.
His research interests include microelectromechanical systems (MEMS) for cell separation.
We used a FACS machine for cell separation in this study.
Various methods have been used for cell separation.
In particular, microfluidic technologies for blood cell separation and plasma isolation are significant for clinical applications.
For PBMC, cell separation procedures were started within one hour from the time the specimens were drawn.
Prior to vaccination, 20 ml of heparinised peripheral blood was taken from all animals for T cell separation.
It's why we also have strict protocols for cell fires.
However, for reasons stated above, it is possible that the integrity/cell specificity for this particular mRNA was not maintained during the cell separation protocols utilized for those databases, leading to conflicting findings.
The proportion of basal cells increased in Wnt1-induced hyperplastic populations, as observed before using a modified cell separation protocol (Fig. S2 [10]).
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