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Total RNA were isolated from cells using Trizol reagent (Invitrogen) according to standard protocols [ 40] and RNA concentrations were determined by UV absorption at 260 nm.
DNA concentrations were determined by UV absorption.
The concentration of MVs was determined by adding a known concentration of calibrated beads (AccuCheck, Life Technologies) according to the manufacturer's protocol.
We found a significant difference in lead dust concentrations determined by the three protocols (F = 4.619; 2,27 degrees of freedom; p = 0.019).
Protein concentration was determined by the BCA protocol (Pierce).
Total proteins were prepared using the glass beads protocol, and the protein concentrations were determined by Bradford (Bio-Rad Laboratories InCA, HercUSAs, CA, USA).
Protein concentrations were determined by the BCA protocol (Beyotime).
Protein concentrations were determined by the Lowry protocol using the Bio-Rad D C Protein Assay.
The requirements for qualifying in concentrations are determined by the committee member representing each concentration.
After which, the supernatants containing leached-out GLP-1C were collected and their concentrations determined by fluoraldehyde reagent according to manufacturer's protocol.
Protein concentration was determined by BCA assay, following the manufacturer's protocol (Pierce ,micro BCA kit).
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