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A novel structure-based virtual screening protocol comprised of e-pharmacophore models and virtual screening workflow was used to identify nine compounds from a commercial database containing 2.84 million compounds.
An orthogonal stability-indicating assay protocol comprised of SE-HPLC, RP-HPLC, ELISA and SDS-PAGE was developed and validated to investigate the effect of further glycosylation of DA on the pattern and kinetics of degradation.
The protocol comprised of two experimental periods of two weeks each with a wash-out period of two weeks in between (Figure 1).
The protocol comprised of alternating 12 s periods of movement and 'rest'rest
The protocol comprised of alternating 12-s periods of movement and 'rest', with each condition repeated four times, in a counterbalanced manner.
The thermal cycling protocol comprised of initial denaturation at 95 °C for 10 min, followed by 40 cycles of denaturation at 95 °C for 10 s and annealing/extension at 60 °C for 1 min.
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The suggested protocol comprises of network synchronization and data transmission phases [58].
The suggested BodyQoS protocol comprises of an Admission Control, QoS scheduler and Virtual MAC (VMAC) [61].
The suggested Superframe structure of MAC protocol comprises of a beacon, emergency access period (EAP), normal access period (NAP), guaranteed access period (GAP) and acknowledgement [56].
In general, the P-ERM protocol comprises of two phases (Table 5), the construction of the partial conformation (cf. Figure 4) and the generation of the candidate sequences (using multiSrch) that conform to the partial conformation.
The protocol comprises of two sets of enzymatic reactions (Supplementary Material, Table S3); in Round I, the sequenase enzyme is used to extend randomly annealed primers (Primer A) and to generate templates for subsequent PCR.
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