Up-to-date there is no consensus about the evaluation protocol applied for HER2 overexpression in EOC.
PJH designed the trial, was responsible for the development of the protocol, applied for a grant and has overall responsibility for the trial.
FvdV, MW, and BMW designed the trial, were responsible for the development of the protocol, applied for a grant and have overall responsibility for the trial.
The PF describes protocols used and an APF details the procedure as well as steps taken with all of the inputs and outputs of each protocol applied for the submitted experiment.
FvdV, MW, PH, GO, MJN and BWM designed the trial, were responsible for the development of the protocol, applied for a grant and have overall responsibility for the trial.
Further, the protocol applied for the assay was the same as for using the in situ PLA on ethanol fixed cells, i.e. not optimized, but the observation of signals is an important step in the development since it opens up for fast investigation of many clinical tumor samples available on TMAs in the future.
Although glutamate, aspartate and imidazole improved the specificity of the original protocol applied for casein tryptic peptides, they were not able to compete with non-specific peptides derived from standard proteins, and so a number of non-phosphorylated peptides were also present in the eluate (Sugiyama et al. 2007).
Controversy exists for protocols applied for the management of these serious injuries.
In February 2005, a working group was therefore founded under the aegis of the Association for Immunotherapy of Cancer ("CIMT") in order to compare techniques and protocols applied for the enumeration of antigen-specific T-cell responses.
The OA chondrocytes were prepared and expanded under the same good manufacturing practice protocols applied for ACT, except that autologous serum was not available from OA patients due to the regulations imposed by the local ethics committee.
