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Therefore, it was the purpose of the present study to evaluate the possibility to establish a typical freezing protocol applicable to a standard freeze-drying unit in combination with an adequate choice of cryoprotective excipients and steric stabilizers to preserve the original particle size distribution.
Consequently, this aromatization reaction continues to attract the attention of researchers to establish a general protocol applicable to a wide range of 1,4-dihydropyridines.
We have created a consistent microarray annotation protocol applicable to all of the major array manufacturers.
This is the first report of a robust RNA isolation protocol applicable to diverse grapevine tissues.
Therefore, we systematically engineered, optimized and refined a protocol applicable to human plasma.
Using an established protocol applicable to metacestode vesicles, we also investigated emir2 expression by in situ hybridisation.
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NSL is an object-oriented language offering object-oriented protocols applicable to all levels of neural simulation.
An average cost optimality criterion is employed to obtain a number of optimal protocols applicable to networks with different computational capabilities.
For that reason, the search for specific sequence-enrichment protocols applicable to massive parallel sequencing has been an important goal during these years.
There have been several recent papers detailing ChIP protocols applicable to low cell numbers (down to 100 cells) based on the inclusion of carrier DNA [ 2] or improvements in the efficiency of the immunoprecipitations themselves [ 3- 7].
To further evaluate whether the developed protocol is applicable to profile TRB repertoire of clinical samples, we tested the protocol on several blood samples from patients with malignant and benign meningiomas (Additional file 1: Tables 1).
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