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The luciferase activity of the p21Waf1 promoter reporter was determined using the Dual-Luciferase Reporter accordingtom (Promega) according thethe provided protocol, and normalized to the corresponding Renilla luciferase reporter activity.
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The relative level of expression of either the mouse or human Pol β mRNA was determined for each sample and normalized to the expression of mouse β-actin using the ΔΔCT protocol, as described in the Methods section.
All spectra were recorded at room temperature and normalized to the incident photon flux.
The mean Ct value was calculated for each gene and normalized to the endogenous control.
Cherry percentages were monitored for 5 days and normalized to the Cherry fraction at day 1 (100).
EGFP plasmids were purified using the Qiagen Plasmid Purification Kit (Qiagen, Valencia, CA) according to the manufacturer's protocols and normalized to 200 ng/μl.
After an additional 3 hours of incubation, β-galactosidase activities were measured using standard protocols and normalized to cell densities (OD600).
(A) Cell survival was estimated by the XTT assay and normalized to untreated control wells.
Cell survival was estimated by the XTT assay and normalized to untreated control wells.
cTnI concentrations were measured utilizing the i-STAT System and normalized to left ventricular mass.
The spectra were blank subtracted and normalized to molar ellipticity.
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