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In paragraph Permitted protocol alterations regarding FDG activity detailed recommendations and permitted alterations of the administration protocol are given, including those for systems that apply a 50% bed overlap.
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In the analysis these individuals were treated as members of the intervention arm (treatment allocated) because they received the intervention but we also performed sensitivity analyses in which we analysed these individuals according to their randomisation to account for this protocol alteration.
Establishing a protocol-alteration policy for your lab is a good idea.
All cohorts experienced changes in appetite and behavior during the acute phase post infection up to d14; however, due to the intense tissue sampling protocol these alterations could not definitively be ascribed to WNV infection.
Five micrograms of starting total RNA was used in conjunction with the Invitrogen I-SAGE Long kit and protocol with alterations [ 24].
LongSAGE[ 25] libraries were constructed with 5 μg of total RNA using the Invitrogen I-SAGE Long kit and protocol with alterations as previously published[ 35].
It could be supposed that in patients with extra GI neoplasms, such as breast cancer, the FEC60 protocol induces alterations in the intestinal permeability associated with modifications in the levels of those hormones regulating the growth and physiology of intestinal membranes (such as GLP-2, ghrelin and, to a less extent, EGF).
In this protocol, DNA alteration that occurs in a bird's offspring is determined by rotating the roulette wheels of the Gamete Mutation Box (GMB), which consists of a short DNA sequence and two roulette wheels.
Adjustments to the published protocol included alteration in fragmentation, cDNA synthesis and adapter ligation.
As possible MDCT device- and protocol-dependent alterations have been addressed, comparisons of MDCT- with HR-pQCT-derived structure measures are to be discussed next.
Immunohistochemical analysis was performed using the labelled streptavidin/biotin-based LSAB + ™ (Labeled Streptavidin Biotin) system/HRP kit (DAKO Australia) according to the manufacturer's protocol, with minor alterations.
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