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Nuclear fractions were prepared from joint tissue using a nuclear extraction kit according to the manufacturer's protocol (Active Motif, Carlsbad, CA).
DNA-binding activity of NF-κB p65 was then measured using microplate-based ELISA according to the manufacturer's protocol (Active Motif).
This illustrates another important feature of our FAST-NMR protocol; active site similarity is a useful tool to predict potential side effects due to off target inhibition, in addition to predicting potential drug leads.
Nuclear extraction was performed according to the manufacturer's protocol (Active Motif, Carlsbad, CA, USA) and the protein concentration was determined using the BCA method.
Whole cell lysis and protein isolation was performed according to protocol (Active Motif) and protein concentration was measured by BCA assay (Thermo Scientific).
Subsequently, the cells were washed twice in phosphate-buffered saline (PBS) and treated with lysis buffer and the extraction of transcription factors from the nucleus was performed according to the manufacturer's protocol (Active Motif, Seoul, Korea).
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All specimens were fixed in formalin and paraffin-embedded using the standard protocols active in the participating centers.
Depending on various study protocols active at each time, the drugs and administration schedule differed (Table 1).
Research done by Bhanage and Zhang [8] to enable a power efficient reading protocol for active RFID shows interesting results.
Li et al. [9] suggest a DCMA (Dual Channel Multiple access) protocol for active RFID where long information packets are used.
To support this form of communication, it is required that a unicast VANET ad hoc networking protocol is active on every CCU.
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