Sentence examples for proteins were seeded from inspiring English sources

Cells, which had been suspended from confluent monolayers by trypsinization followed by inhibition of trypsin with fetal calf serum and repeated centrifugation alternating with suspension in PBS to remove serum proteins, were seeded in the coated wells at 37°C in Dulbecco's modification of Eagle's medium (DMEM, Cellgro Mediatech, VA) supplemented with 0.2% fatty acid-poor BSA.

HeLa or MDA-MB-231 cells transiently transfected to express the GFP-actin fusion protein were seeded on 4-well LabTek chambers that had been coated with 10 μg mL−1 of fibronectin.

Immunohistochemistry studies confirmed results observed by Western blots ruling out the possibility that, although the same amount of protein was seeded, fewer epithelial cells expressing high levels of ER-α were masked.

For cell surface expression of receptor proteins, cells were seeded as before, harvested, and rinsed twice in PBS/0.2%BSA/0.22% Sodium Azide.

For confirmation of downregulation of BRAF protein, cells were seeded and transfected as indicated above and processed at 24, 48 and 72 hours.

For protein measurement, cells were seeded into 10-cm dishes, and treated with drugs or H2O2.

To measure the rate of 35S-Met incorporation into protein, 60,000 cells/well were seeded in a 24-well plate.

For RNA and protein collection, cells were seeded in 100 mm dishes at 0.5-2×106 cells and grown for 1 2 days (60 80% confluency).

For larger scale protein production, cells were seeded in 750 ml bottles at 2 × 107 cells/bottle.

To examine the effect of emodin on Runx2 and PPARγ protein, primary BMSCs were seeded in 6-well plates at a density of 5 × 10 cells/well.

For cDNA or protein collection experiments cells were seeded in 6-well plates at 2 × 105 cells/well.