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c Whole-cell proteins were assayed by western blotting.
The interactions between the tested proteins were assayed by monitoring yeast colonies growth on selective medium DOB-Leu-Trp-His to detect the activation of the His reporter gene.
After washing, captured proteins were assayed by WB.
Proteins were assayed by the BioRad approach (BioRad, München, Germany).
(A ) Immunoprecipitation was performed with streptavidin beads from biotin-tagged REST ESCs or BirA control ESCs and the demarcated proteins were assayed by Western blot.
GTP-hydrolysis activities of recombinant Rab and HRas proteins were assayed by quantitating released free phosphate molecules, using the Malachite Green-based reagent Biomol Green (Enzo Life Sciences).
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Necrosis inducing activity of purified proteins was assayed by infiltration into tomato or tobacco young leaves.
The expression levels of nm23-H1 protein were assayed by the immunocytochemical method after transfection for 48 h.
RCC10 cell lines were treated with cycloheximide for given amounts of time and levels of VHL protein were assayed by western blotting.
The effects of MU on the expression levels of Akt and phospho-Akt (p-Akt) protein were assayed by western blot analysis.
Routine blood tests, including lipid profile, blood cell count, glucose, serum creatinine and high-sensitivity C-reactive protein, were assayed by automated laboratory techniques.
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