While comparisons can be performed in well-defined conditions (e.g., to compare two formulations of the same protein at the same concentration), more quantitative measurements of particulate matter <10 μm in protein formulations can only be performed by optical techniques at protein concentrations below 10 mg/ml.
In addition, soluble HIV-1 Vpr protein at a subneurotoxic concentration (50 nM) together with HCV core protein (10 and 100 nM) caused significantly increased HFN injury (Figure 6C) indicated by reduced β-III-tubulin immunodetection, compared with HFNs exposed to either HIV-1 Vpr or HCV core proteins at the same concentrations (p<0.05).
C3b-biotin (300μg/ml), C5 (150μg/ml) or a mixture of both proteins at the same concentrations were incubated for 1 hour at 4°C in VBS with 2.5mM MgCl2 and subsequently analysed on a Superdex 200 10/300 GL column (100μl injection volume).
Using narrow-mouth pipet tip, adjust proteins to the same concentration, and spot 2 μl of samples onto the nitrocellulose membrane at the center of the grid.
For competitive binding assays, all ligands were added to a protein solution at the same concentration.
MBP-MBD5, and MBP-MBD6 were highly expressed and soluble; MBP-MeCP2 was expressed less efficiently (Figure 6E); the three proteins were used at the same concentration to test interaction with the CpG-rich probe SL3.
The ligated complex catalyzes GTP hydrolysis at much higher rates than the two individual proteins mixed at the same concentrations, indicating enhanced formation of the catalytically competent plexin/Rap complex when the two proteins are tethered.
(C ) Comparison of the GTP hydrolysis activity between the ligated complex and the individual PlexinC1cyto and Rap1B proteins mixed at the same concentrations.
Five different proteins were mixed together at the same concentration (20 μM) to find the optimum matrix and deposition method for pure protein analysis.
Although in principle the model can represent cells where proteins are present at different concentrations and interact with different affinities, here we focus on the topological properties of the network and we use a simple model where proteins are all present at the same concentration and interact with the same "strength".
However, survivin recombinant protein (BIRC5) at the same concentrations was not endocytosed.
