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Treatments had similar fat, protein, volume and weight.
Sorbitol was more effective than glycerol at decreasing the protein volume at high cosolvent concentrations, which may be because glycerol has some surface activity and may therefore accumulate around hydrophobic regions on the protein surface.
At the same time, application of supersharp AFM probes allows to measure protein volume more correctly.
It has been observed that the active site volume scales with the protein size whereas there is little correlation between protein volume and ligand volume [26].
In the uncharged ASIC1, although the influence of the atomic charges of the proteins is absent, the protein volume still prevents solvation of the 6 titratable acidic residues.
These steps were taken to ensure that changes in levels of protein expression were not simply due to differences in overall protein volume or spotting variances.
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Atomic force microscopy can potentially provide information on protein volumes, shapes, and interactions but is susceptible to variable tip-induced artifacts.
Together with the protein of interest, we coadsorb a DNA molecule and use it as a fiducial marker to account for tip-induced artifacts that affect both protein and DNA, allowing normalization of protein volumes from images taken on different days and with different tips.
Moreover, miniaturization of SAEs can further decrease protein volumes necessary for the analysis.
The estimate relies on knowledge of the protein mass per unit volume (denoted by cp).
The standard probe tip (R ~ 10 20 nm) broadening effect leads to substantial overestimation of measured protein's volume.
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