Sentence examples for protein separate from inspiring English sources

Integrating the knowledge about the binding modes of ligands in different conformational states of the protein, separate pharmacophore models were generated and used for virtual screening to explore potential novel hits.

Rabbits were immunized subcutaneously with 3 doses containing 50 µg of each purified recombinant protein separate, or with a cocktail composed of 30 µg of each recombinant protein, emulsified with Complete Freund's Adjuvant (first dose) and two subsequent booster injections emulsified in Incomplete Freund's Adjuvant at 3-week intervals.

Using retroviral gene delivery to PC-3M PCa cells, we expressed luciferase-only, enforced CD44s re-expression as a fusion protein with luciferase at its C-terminus or as a protein separate from luciferase, or knocked down CD44v7-10 by RNAi.

MBP/endo was also cleaved (Figure 2 lane 3, 4 and 5), resulting in an MBP (50 kDa) protein separated from endochitinase (39 kDa) protein.

Usually, 75-100μg of protein separated by SDS-PAGE using 4-20% PAGE Novex-ready gels (Invitrogen).

Proteins separated by 2-DE were identified by peptide mass fingerprinting (PMF) after in-gel digestion.

Purity was ascertained by Coomassie staining of proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Proteins separated by SDS-PAGE were electrophoretically transferred to PVDF membranes (Millipore).

Cytoplasmic proteins separated on 10% SDS-polyacrylamide gels were transfered on Hybond ECL membranes (GE Healthcare).

Supernatants were then concentrated 10 fold and proteins separated by SDS-PAGE.

Proteins separated by SDS were stained with Coomassie Brilliant Blue R250.