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Trypsin was added in a 1 50 trypsin:protein ratio for overnight digestion at 37 °C.
Moreover, dietary composition fluctuated in both groups and differences in the carbohydrate to protein ratio were not maintained.
Protein ratio fold-changes greater or less than 2 s.d. were assigned as significant for up- or downregulation respectively.
The next morning, trypsin (Promega) was added to a final 1 100 enzyme:protein ratio for 6 h at 37 °C.
375 ng of trypsin was added to the protein extract in a 1 40 enzyme to protein ratio and incubated for 16 hours at 37 °C.
Two hundred micrograms of protein were diluted to 2 M urea and digested with LysC (Wako) in a 1 100 enzyme:protein ratio overnight at 30 °C.
Similar(25)
As yet no convincing evidence exists that a special carbohydrate-to-protein ratio makes a noticeable difference in muscle protein maintenance after exercise.
The CHO-to-protein ratio in CEP was 2 1.
A dye-to-protein ratio of ~2.5 was measured from absorbance at 280 nm (for Tf) and 494 nm (for AF488) on a NanoDrop 1000 spectrophotometer.
Therefore, theoretically, vegetarian diet has a lower phosphate-to-protein ratio leading to decreased phosphorus absorption in comparison with animal-based diet.
The fat-to-protein ratio was <1 and similar among all treatments.
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