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The relevance of Antigen 85 protein quantities and vaccine efficacy remains to be defined.
The equilibrium is such that at physiological pH the active, read-through permissive conformation is populated at approximately 6%: a level that correlates with in vivo protein quantities.
Peshkin then repeated this experiment, this time measuring protein levels only at the moment of fertilization and excluding the protein quantities throughout development.
The algorithm crunched the information from these two data sets and then determined the protein quantities throughout each stage of early development.
Our data demonstrates that the greatest variation in Antigen 85 is ascribed to protein quantities, whereas few transcriptional differences were found.
In contrast, no such relationship between individual protein quantities was observed, and in the case of Antigen85 B, this variation even extends within biological replicates of individual isolates.
Assays are adaptable to most biological matrices, highly multiplexible and require small protein quantities.
Vogel and Marcotte [33] suggested that the correlation between mRNA and protein quantities is approximately 40%.
In this study mRNA levels are analysed, not protein quantities.
All protein quantities in the proposed studies were measured by the Bradford method (Bio-Rad).
Protein quantities were estimated using the bicinchoninic acid (BCA) protein assay (Pierce, Rockford, IL).
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