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The most widely used technique for protein quantification is ELISA.
With the standard ADH1 as references, protein quantification level was exported in femtomol and nanogram.
Proteins with no missing values in three replicates were accepted in the protein quantification analysis.
on the results of these traditional methods of protein quantification (Olson and Markwell 2007).
Protein quantification was performed by Reporter Ions Quantifier with the TMT sixplex method on Protein Discoverer.
Spectrophotometric soluble protein quantification was made as described in Bradford [36].
Preliminary bioassays revealed that some fractions showed low protein quantification and no antibacterial activity.
Protein quantification was performed by using Qubit-Invitrogen™ according to manufacturer's instructions.
Protein quantification was performed using the reporter ions quantifier with the TMT 6-plex method.
The protein extraction was quantified using BCA protein quantification kit (Dingguochangsheng Biotech Co. Ltd. Beijing, China).
BCA kit purchased from Thermo Fisher Scientific was used for protein quantification.
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