Biologically independent experiments were performed on independently made protein preparations.
All protein preparations were >95% pure as assessed by SDS-PAGE and Coomassie staining.
The experiments were repeated three times using different protein preparations and the most typical results are presented.
The proposed protocol is simple, fast, inexpensive and also applicable to protein preparations of other plants.
Protein aggregation is a major issue affecting the long-term stability of protein preparations.
Moore, R. A., Timmes, A., Wilmarth, P. A. & Priola, S. A. Comparative profiling of highly enriched 22L and Chandler mouse scrapie prion protein preparations.
Ostuni, M. A., Iatmanen, S., Teboul, D., Robert, J. C. & Lacapere, J. J. Characterization of membrane protein preparations: measurement of detergent content and ligand binding after proteoliposomes reconstitution.
Under these conditions, negative stain TEM of the protein preparations revealed globular particles of various sizes that had no recognizable structure (Fig. 2A).
In phase 1, the patients eat high-biological-value protein preparations five times a day, and vegetables with low glycemic index.
To test both recombinant protein preparations for endotoxin contamination, we utilized two assays: an endpoint chromogenic LAL assay and the EndoZyme recombinant Factor C assay.
Proteins were analyzed via SDS gel electrophoresis in order to detect major impurities in protein preparations.
