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The obtained crystal structure was first prepared using Protein preparation utility of Schrodinger [ 19- 21].
All employed PDB structures were prepared with the protein preparation wizard of Schrödinger 2010 [24].
All the above targets were prepared using the "Protein Preparation Wizard" module of the Maestro software [14].
In Glide, the protein structure was prepared using the Protein Preparation and Grid Generation modules, with all default settings.
The protein 3D structures were prepared in the Protein Preparation Wizard.
Protein structures for binding-site analysis were prepared using the protein preparation function in Maestro (Schrödinger).
The protein (PDB: 2J5F) was prepared using the Protein Preparation Wizard.
To obtain a satisfactory starting structure for following studies, the Kv7.2 protein was prepared using the Schrodinger Protein Preparation Wizard65.
The protein was further prepared using Schrodinger's protein preparation wizard [ 36].
This processed protein was further prepared using Schrödinger's protein preparation wizard [ 17].
The protein was then prepared for docking using Schrödinger's protein preparation wizard [ 16].
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