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For sub-50 nm resolution, protocols often exhibit slow patterning speeds, complex fabrication, or poor control of protein positioning.
Finally, a SCA11-associated mutation of TTBK2 resulted in localization of the protein to the nucleus [ 34], an aberrant protein positioning with unknown effects on cells.
Approximately 85% of recombinant protein positioning on the surface of E. coil cells exhibited high activity and stability.
Several downregulated GO classes are involved in protein turnover including endoplasmic reticulum/ER, isomerase activity, amino acid derivative biosynthesis, protein positioning, and the proteasome.
Consistent with this model, downregulated GO categories in our data include several involved in protein turnover including endoplasmic reticulum/ER, isomerase activity, amino acid derivative biosynthesis, proteasome core complex, and protein positioning and transport.
Additionally, while a reduction of GpIbα led to some normalization of cytokinesis protein localization in cancer cells, GpIbα, F-actin and filamin A remained mislocalized in many HeLa cells after knockdown of GpIbα demonstrating that other factors also interfere with cytokinesis protein positioning in these cells.
Similar(52)
The poly-peptide fold from residue 114 to residue 264 is homologous to that of chymotrypsin-like serine proteinases with catalytic residues His 141, Asp 163 and Ser 215 of the core protein positioned as in other serine proteinases.
Of the fur genes from 20 transformants sequenced, ten contained single substitutions, six of which affected just one position in Fur protein (position 41); the other four transformants contained single mutations affecting protein positions 40, 41, 57 and 95.
This analysis showed that all seven neutralising epitopes critical for both structure and function of the protein positioned at individual residues D72, E74, A75, K78, A79, L343 and the stretch of amino acids 157ILRLKESIAATNEAVHEVTDG171, are conserved [40], [41], [42].
As a result, the proteins become focused into sharp stationary bands with each protein positioned at a point in the pH gradient corresponding to its pI.
FGFs bind and dimerize the extracellular domains of FGFRs in concert with heparan sulfate glycosaminoglycans or single-pass Klotho coreceptor proteins positioning the cytoplasmic kinase domains in proper proximity/orientation for transphosphorylation on A-loop tyrosines.
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