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OL consists of lamellar protein, oriented normal to dorsal edge.
For a protein oriented exon taxonomy to be complete it must describe partially and wholly untranslated exons (henceforth uexons).
Given the lack of thorough protein oriented exon taxonomies the need for a new categorization method was obvious.
Unlike the states used in some automated genefinders [ 3, 5], the POEM attempts to identify every possible protein oriented exon type, especially those which span CDS boundaries.
The protein oriented exon monikers (POEM) is based on dimensions known to reflect statistical features and attempts to include every possible exon category.
The construct ECFP-SQS1 codes for the ER transmembrane domain of squalene synthase (SQS1) of Arabidopsis thaliana [ 22] fused towards N-terminus to a fluorescent protein oriented at the cytoplasmic side.
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Separated Local Field (SLF) spectroscopy is a powerful tool for the determination of structure and dynamics of oriented systems such as membrane proteins oriented in lipid bilayers and liquid crystals.
In this work, we describe specially designed, large volume, low-E 15N 1H solid-state NMR probes developed for 600 and 900 MHz PISEMA studies of dilute membrane proteins oriented in hydrated and dielectrically lossy lipid bilayers.
This is a simple and helpful method to localize Cys residues in regions of membrane proteins oriented toward the cytosolic side [ 58].
With FKBP12 as the model target protein, the ligand binding pocket-oriented labeling reagent could label intracellular protein, whereas protein surface-oriented reagent was ineffective for labeling in living cells, partially because of the intracellular protein fluctuation under the macromolecular crowding effects.
Specular x-ray reflectivity shows that the amphiphilic protein orients vectorially in Langmuir monolayers at higher surface pressure with its long axis perpendicular to the interface, and that it possesses a length consistent with its design.
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