The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytological marker to monitor protein localisation in live cells.
In the present study, balance between mRNA expression of APP and a potent amyloid degradation enzyme, neprilysin (NEP), as well as protein localisation of APP and NEP were analysed.
The RNA and protein localisation data leave us with a number of questions concerning synaptic connectivity in the insect brain.
Protein localisation is certainly important, but it also interesting to classify the described proteins according to their functions.
This suggests that the few amino acid encoded by attB1/B2 recombination sites do not significantly interfere with protein localisation.
Significantly, our observations suggest that this event leads to the dissociation of Armadillo and E-Cadherin protein localisation, and accumulation of punctate Armadillo in a subapical compartment.
Fluorescent reporters have been used to investigate transcriptional activity, for protein localisation, to screen microbial population dynamics in situ, and for comparative genomic, transcriptomic, and proteomic studies.
However, following activation by xanthurenic acid, rapid changes in protein localisation and abundance occurred, and stable sex ratios could no longer be derived from IFAT studies.
To quantify these findings, the number of cells containing detectable aggregates or showing a dispersed pattern of wild-type protein localisation was determined following shR-P16 treatment and compared to that seen with the non-specific control (Figure 5C).
In these conditions after 24 hr of starvation the protein localisation was cytosolic and, upon stimulation with serum (10%) or FGF4 (80 ng/ml), ERK1-GFP translocated in the nucleus (figure 1).
During inhibition, the phosphorylation rate drops to 0 and we can fit the model prediction of the actual time course of protein localisation to obtain an estimate of the de-phosphorylation rate.
