The aim of our work was to evaluate SOD isoforms' activity and protein level changes in liver tumors.
In terms of the extent of protein level changes, the high salt treatment appears to have a more marked effect on tertiary proteins (Fig. 1C).
In total, 274 proteins, mostly membrane proteins, were identified, and 50 proteins exhibited differential protein level changes, with 40 proteins increased and 10 decreased.
Treating cells with the ERRα antagonist for 12, 24, or 48 hours yielded negligible ERα protein level changes (Fig. 4B).
Furthermore, β-catenin and GATA4 protein level changes were dependent on the concentration of antisense NKX2-5 (AdXKN) used in the experiments (Fig. 2C).
In other words, a large number of protein level changes may occur that would not be apparent from the study of mRNA data alone.
In addition to substitutions at the protein level, changes in gene regulation are likely to underlie many phenotypes of interest, including human-specific adaptations and diseases [1] [8].
Moreover, the protein level changes of the target genes showed that both SeTre-1 and SeTre-2 decreased immune signals in response to the dsRNAs injection from 48 hours post-injection, especially at 72 hours (Figure 5D).
Moreover, in western blot analysis result, the protein level changes of the target genes showed that both SeEcR-A and SeEcR-B1 were not influenced at 12 hours after dsRNA injection, but decreased immune signals are detected in response to the dsEcRcom injection at 36 hours post-injection (Fig. 3E).
During the malignant progression of the MOSE cells, the microfilament, microtubule and intermediate filament systems became sequentially disorganized, highlighted by i) distinct protein level changes, ii) the significant loss of polymerized F-actin, and iii) the decreased capacity for formation of focal adhesions.
