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The amount Of M2 protein incorporated into VLPs was estimated to be approximately 1% of the total protein (Fig. 1A).
Analysis of the N protein in the clarified supernatants showed the detection of a single N protein species that represents the N protein incorporated into the virion particles in cells treated with DMSO alone (Fig. 6, bottom panel, lanes 6 10).
As a control, blank microvesicles (no protein incorporated; cMV) were used, prepared and handled exactly as the A2MG-MV but devoid of any protein, and are termed cMV.
These spectra, which have been normalized by total spin number, are shown for the protein incorporated into POPC/POPS bilayers and DPC micelles.
For the analyte proteins, the His-tag was cleaved and the protein incorporated into the running buffer over a range of concentrations (100 2000 nM).
Thus the expressed protein incorporated the amino acid residues LEHHHHHH at the C-terminus in addition to the full-length sequence of human CaM.
Similar(46)
Thus, the FRAP data in Fig. 5B suggest wt protein incorporates into larger soluble complexes in the presence of mHttex1.
Although we do not know whether this fusion protein incorporates within polymers of the endogenous Sup35p, the fusion protein is induced to form prions by the endogenous [PSI+] and retains the propagation characteristics of the prions that induce it [60].
A predicted protein incorporating the DUF1313 domain is also present in C. reinhardtii [Additional file 1].
This scenario would be similar to that in yeast, where only a single heterotrimeric G protein, incorporating the Gβ protein Ste4, has a specialized role in transducing mating type signals from heptahelical mating-factor receptors [ 22].
All H-DARPin proteins incorporated into LVs, albeit at different levels.
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