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The ELISA results for the surface protein in the medium of the recombinant MVA, strongly indicate that the recombinant virus has been successfully obtained.
After 4 days, the concentration of secreted IL-12 proteintein in the medium of the transfected cells was measured using an IL-12 p40 ELISA kit (BioLegend).
Galectin-3-binding protein was the most significantly up-regulated protein in the medium of ACH-2 cells.
The up-regulation of galectin-3-binding protein in the medium of ACH-2 cells suggested that T cells latently infected with HIV could contribute to the elevation of galectin-3-binding protein that was observed in the plasma of HIV+ patients.
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Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7.
The elevated levels of these six proteins in the medium of ACH-2 cells and HIV+ plasma suggested that their up-regulation in the HIV+ plasma might be partially due to HIV latently infected T cells.
Here we found that U18666A treatment significantly increased Il6 expression and Il-6 protein concentrations in the medium of osteoblasts in a concentration-dependent manner (Fig 8C).
Another striking observation is the high apparent abundance of the GPI protein Phr1 in the medium of fluconazole-treated cells, whereas it is not detectable in the medium of single-cell yeast cultures and hyphal cultures.
Based on the known functions of BACE1 and the short list of established β-secretase substrates, we expected to find solely membrane-bound proteins enriched in the medium of BACE1 expressing cells.
The correlation of the proteins observed in the medium of HIV+ T cells and plasma was not clear in the study.
Immunoblotting for MMP-11 protein demonstrated an increase in the secretion of cleaved, active MMP-11 protein in the conditioned medium of 231-Gli1 comparedmpared to the conditioned medium of the vector control cells (Fig. 4b).
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