The Min protein oscillations and Min gradient formation were supported by both profiles.
DOI: http://dx.doi.org/10.7554/eLife.05508.014 As the MPZ− and SogZ+ protein system facilitated interaction and SogZ+ cleavage, we tested the ability of these proteins to rescue Dpp gradient formation in a tld null background.
Glypican Dally and Dlp (Dally-like proteins) are involved in gradient formation by transferring Wg to neighbouring cells, thus inhibiting the binding of Wg to its local receptor for signalling [ 133].
Similarly, ONT1, a secreted Olfactomedin-class protein, facilitates Chordin-BMP1 interaction by independently recruiting both proteins, resulting in robust BMP gradient formation during embryonic DV patterning.
Since nuclear localization of a protein is insufficient for proper gradient formation in embryos [44], it is evident that additional properties of Bcd, such as its DNA binding function as modeled in our study, are likely to play an important role in this process.
In contrast, simple physical parameters, such as electrostatic interactions between the membrane and the proteins, are strong determinants for gradient formation.
There is good evidence that the formation of the protein gradient of the morphogen Bicoid (Bcd) takes place by means of these two processes.
In conclusion, we have uncovered an important function of the membrane microdomain-scaffolding protein reggie-1 in secretion and gradient formation of the lipid-modified Wg and Hh morphogens in Drosophila.
Based on the observed dynamic distributions of bcd mRNA in early embryos, it was suggested that the shape and formation of the Bcd protein gradient are dictated by bcd mRNA distributions.
Small variations in the corresponding shapes of the compartments occurred during the fabrication process, but still supported oscillations and gradient formation of the Min proteins.
