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Studies of gene expression and function coupled with studies of protein (coding) and regulatory (noncoding) sequence of the genes in the different species will allow for correlations between genetic changes with important evolutionary events for the diversification and adaptation of the group.
Protein functions are tightly coupled with protein structures and structures are much more conserved than sequences [ 13].
Low dietary protein coupled with exposure to this metal induces more severe changes, including biochemical defects, structural disorders, and altered physiologic functions.
The identification of the biochemical activities of many effector proteins, coupled with a better understanding of their potential contribution to pathogenesis, has revealed common themes in the evolutionary design and function of these remarkable bacterial proteins.
Chemical cross-linking of proteins coupled with mass spectrometry analysis.
The structural similarity to RNA-binding proteins, coupled with the observation that Alba can bind to RNA in vivo and in vitro [ 8], has prompted the suggestion that Alba may also function as an RNA-binding protein in vivo [ 9].
Likewise, three-dimensional models of proteins are used to gain insight into protein function and to assist with drug design.
Bilayer-mediated alterations of membrane protein function arise because the proteins are coupled to the bilayer through hydrophobic interactions.
Site-directed or deletion mutagenesis and protein domain-swapping experiments, sometimes coupled with epitope- or GFP (green fluorescent protein -tagging of recombinant channels, have revealed key amino acid residues and domains influencing TRprotein -taggingnctiof (Table 1) [ 25– 29].
Genetic screening coupled with gene-editing protein function testing is an effective and reliable method by which causative gene mutations of MODY can be identified.
The slow glutamate responses are mediated by metabotropic glutamate receptors (mGluRs) through G-protein coupling with numerous intracellular signalling cascades that can modulate ionotropic receptor function [ 1].
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