Sentence examples for protein expression vector from inspiring English sources

HIV vector particles expressing GFP following integration were generated by cotransfecting HEK293T (ATCC, Manassas, VA) cells with a VSVG envelope protein expression vector, a packaging vector, and a plasmid expressing genomic HIV RNA including a CMV promoter that drives expression of GFP as described [38].

It is based on the constitutive expression of green fluorescent protein (GFP) mediated by the bacterial protein expression vector pGFPuv (Clontech, Palo Alto, USA).

We have designed a new protein expression vector that fuses the target protein to the N-terminus of the integral membrane protein, PagP.

These E. coli strains and the protein expression vector pET-28a were purchased from invitrogen (Carlsbad, CA, USA).

In addition, we incorporated a loxP site into a GST fusion protein expression vector to enable high-throughput cloning of cDNAs encoding potential sumoylation targets.

The open reading frame of TRAF4 was cloned into the pEYFP-N1 vector (Clontech) to generate a TRAF4-EYFP fusion protein expression vector.

Protein expression vectors designed for large-scale protein production often include one or more specific tags to such transcripts, to simplify the purification of the targeted protein.

(D) Either an EGFP-NOC4L or NOC4L-EGFP protein expression vectors were transiently transfected into Hela cells.

Bacterial BL21 competent cells were transformed with GST and GST-WT1 fusion protein expression vectors, respectively.

Further, three of the predicted peptides were cloned into protein expression vectors to test the biological activity.

Microarray protein expression vectors were prepared in 3× standard saline citrate (SSC) in a 384-well plate (Genetix, Boston, MA) and arrayed on nitrocellulose coated "Fast Slides" (Schleicher & Schuell BioScience, Keene, NH) using a Microgrid II microarray robot at 50% humidity.