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Our study is the first to dissect the protein expression response of chloroquine and artemisinin treated Plasmodium falciparum cells on the proteome level.
This temporal correlation between mRNA and protein expression is not specific to rapamycin, since oxidative stress also induces rapid transcriptional changes which peak at 60 min after the treatment and decay quickly, while the protein expression response is much slower [ 38].
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In this study, we investigated the protein expression responses of R. erythropolis strains and found that isocitrate lyase production in R. erythropolis PR4 (ICLRe) was induced by methanol.
They found that the overall correlation between transcriptional and protein expression responses was low, and suggest that this reflects pervasive post-transcriptional regulation.
These subtle differences in mRNA and protein expression responses to VEGF and PlGF suggest that post-transcriptional mechanisms, such as regulation by microRNAs 26, may be operative.
In addition, we only assessed expression of insulin signalling proteins in the basal state – i.e. signalling protein expression in response to fasting insulin concentrations – and in response to maximal insulin stimulation.
Increased insulin receptor protein expression in response to RT might be another important adaptation responsible for the insulin-sensitizing effect of training [ 39].
Functional elements in the 5'-UTR such as internal ribosome entry site (IRES), upstream ORF and iron responsive element (IRE) serve to fine tune protein expression in response to cellular requirements.
Functional changes in collagen type I protein expression in response to MSC were confirmed by ELISA.
Nevertheless, Rbpj-deficient BMDMs were able to upregulate Arg1 mRNA and protein expression in response to IL-4 stimulation (Fig. 4A and 4B, lanes 6 10).
Estrogenicity was also assessed by the use of transgenic zebrafish embryos that exhibit green fluorescence protein expression in response to aromatase (cyp19a1b) gene induction, with confirmation of results by qPCR [52].
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