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The relative protein expression level was expressed as a ratio over β-actin.
We compared the resulting protein expression level to full length proteins expressed from native genes.
However, at the protein expression level, these differences were modest.
Up-regulation of Bax and down-regulation of Bcl-2 protein expression level confirmed apoptosis.
Protein expression level control is always a great challenge in the field of enzyme evolution.
MKP-1 phosphorylation and protein expression level are differentially altered upon TM and DTT treatment.
The protein expression level was detected at 72 h p.i to compare the maximum yield of EGFP.
Consistently, the HCV NS3 protein expression level in siRBM24-transfected cells was also significantly decreased (Fig. 1G).
Western blot analysis revealed that protein expression level of Svr was significantly decreased in this mutant (svr 1021, Fig. 1B).
In this study, we first identified that the two regulatory elements increased the EGFP protein expression level in the BEVS.
These results indicated that the increased EGFP transcriptional level was the main reason for the increased EGFP protein expression level.
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