Protein estimation of supernatants was carried out using the Bradford assay.
Lysates were incubated at 4°C for 30 min, sonicated (10 pulses 5 sec), centrifuged at 14,0000 g for 10 min, and the protein concentration of the supernatant quantified (BioRad protein estimation kit, Hercules, CA).
Tissue lysates were then centrifuged on a microcentrifuge (14,000 rpm, 20 min, 4°C; Eppendorf, Fremont, CA, USA), the protein content of the supernatant (total muscle extract) was determined with a Micro-BCA protein estimation kit (Pierce, Rockford, IL, USA), and the samples were subjected to protein normalization.
The protein concentration of the supernatant was determined by the Bradford dye-binding assay (Bio-Rad, Hercules, CA).
The protein concentration of the supernatant was determined at intervals.
The protein concentration of the supernatant was determined.
Protein concentration of the supernatant was determined using BCA Protein Assay Kit Piercee).
Protein concentration of the supernatant was determined by Bradford assay.
The protein content of the supernatant was determined with the BCA protein assay (Pierce, Rockford, IL).
The protein concentration of the supernatant was determined using a protein assay kit Bio-Rad Laboratoriess, CA, USA).
