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The most common differences in quantity between the total nitrogen and true protein estimates was 0.3 g/dL (Table 4).
For a query protein, estimates of the conditional probabilities of the protein to be localized to each location, given its amino acid sequence, are determined.
Some researchers presented their estimates of breast milk protein content based on the total nitrogen, assuming that all of the nitrogen represented protein [ 6, 11- 14, 120 23, 122, 123, 125, 128, 128- 135], some presented both protein estimates [ 11- 18, 128 129] 129], while other researchers reported only true protein estimates [ 11- 18, 19, 19, 121- 123, 127- 129, 136- 139].
This implies that a more accurate estimate of the RNA levels is likely to increase the overall correlation, and that the cause of low correlation are mainly caused by variable accuracy on the RNA level and not the protein estimates.
The protein meta-analysis estimates based on total protein were almost uniformly higher than the true protein estimates, which suggest that these two approaches should not be averaged together.
For mRNA estimates, we used a lower baseline setting (T/N fold-change 1.5, instead of 2 in the case of the protein estimates) taking into account the use of the quantitative RT-qPCR method.
Similar(50)
The radius of gyration (R g) of the protein estimated from Guinier analysis is about 19.0 Å.
The yield of the recombinant VEGF165 protein, estimated by SDS-PAGE and Western Blot, was higher when using subsequently lysozyme and sonication treatments, compared to all other methods.
Using a photoaffinity-biotin labelled PBAN analog we demonstrate specific binding of a membrane protein (estimated MW: 50kD) in adult females.
A level of purified scFv-myc-His fusion protein estimated at 40 mg/L was reached at pH 6 and a temperature of 27 °C.
Assays were performed using affinity-purified recombinant protein estimated by SDS-PAGE.
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