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After lysis and protein digestion, DNA was digested in agarose plugs using PciI and labeled by TUNEL.
The critical step in the first two strategies is the protein digestion step, since usually not all peptides of the digest are detected by mass spectrometric analysis.
Proteins in gel bands were reduced, carboxyamidomethylated and digested with Trypsin Gold (Promega) on a robotic platform for protein digestion (MassPREP station, Waters).
Protein digestion and iTRAQ labeling was conducted in 2 technical replicates.
Intestinal amino-oligopeptidase (AOP) plays an essential role in protein digestion.
In general, after baking protein digestion was significantly increased.
Goupil, L. S. et al. Cysteine and aspartyl proteases contribute to protein digestion in the gut of freshwater planaria.
The above results demonstrated that the protein digestion with our novel MoS2-based immobilized trypsin reactor is superior to the conventional protein digestion with free trypsin.
Adibi, S. A. & Mercer, D. W. Protein digestion in human intestine as reflected in luminal, mucosal, and plasma amino acid concentrations after meals.
Several peptides derived from food protein digestion possess regulatory functions that can lead to health promotion.
Therefore, even after total removal of the stomach, protein digestion usually is not impaired.
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