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In another study, Bashor et al. [ 27] constructed a synthetic scaffold protein device to systematically reshape response dynamics of the yeast mating MAPK (mitogen-activated protein kinase) pathway.
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In addition, we discuss the computer-aided design of logical-AND gates and of protein devices in bacteria.
We have recently moved into engineering such structural transformation in RepA-WH1 to build-up synthetic protein devices that allow for customized ligand (DNA -promoted amyloiDNA -promoted
The charge transfer process in protein devices can be engineered by using proteins with different redox centers (metal atoms) and by controlling their orientation in the solid state through different immobilization methods.
Future applications of this transposon mutagenesis approach are expected to simplify the discovery of fragmented protein devices that couple the function of other fragmented proteins to protein protein interactions.
The abstraction hierarchy proposed by Endy [ 12] classifies standard parts in three different layers: parts, which are defined as sequences with basic biological functions (like for example DNA-binding proteins), devices which are combinations of parts with a particular function and systems which are combinations of devices.
It was achieved by a novel approach to proteomic research using a specially developed serum/plasma protein separation device (hollow-fiber-membrane-based device; HFRD, Toray Industry, Tokyo, Japan) and a linked two-dimensional liquid chromatography system (2D LC-MS/MS) [ 10].
The goal of this work was to utilize the naturally derived bioactive polymer hyaluronic acid (HA) to create a combination tissue engineering scaffold and protein delivery device.
Designing a colloidal carrier to serve as a protein delivery device requires an understanding of the effect of different materials on the physicochemical, physiological and toxicological parameters for clinical application.
TTSS is a needle-like protein transport device used by Gram-negative pathogenic bacteria.
This modular machine in the outer membrane receives its substrates via small TIM chaperones that link the protein translocation device (the TOM complex) with the SAM complex [11], [12].
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