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Ripening of barrel-salted herring (Clupea harengus) is evaluated by the use of fluorescence spectroscopy and protein determinations.
The possibility of such ″bottom-up″ architecture mounted with ″top-down″ weight reactor would be most likely extensible and adaptable to protein determinations.
After sonication, 3 µl of total extract were used for protein determinations.
Co-author LW collected drusen used for total protein determinations, and co-author MEC isolated all others.
The resulting pellets were then suspended in fixative solution for electron microscopy analysis or prepared for lipid and protein determinations, as described below.
Protein determinations were performed using the Micro BCA assay (Pierce) and 5 µg of nuclear extract (with protease inhibitors, Roche) plus rERα (170 nM) was used in each lane of a 5% acrylamide gel in TBE buffer.
Similar(29)
Protein concentration was determined by BC assay protein determination kit (Interchim).
Protein concentration was determined using the Bio-Rad protein determination assay reagent.
Purified virus concentrations were determined by using an EZ-Q protein determination kit (Invitrogen).
Protein content of cell lysates was determined using the bicinchoninic acid (BCA) protein determination assay (Pierce Biotechnology, Rockford, IL, USA).
Total protein concentration was determined by the bicinchonic acid method, as mentioned in the Protein determination section above.
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