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Protein concentration was determined by BC assay protein determination kit (Interchim).
Protein concentration was determined using the Bio-Rad protein determination assay reagent.
Purified virus concentrations were determined by using an EZ-Q protein determination kit (Invitrogen).
Protein determination was carried out according to the Bradford assay.
Protein determination was performed using the modified Lowry method [23].
For the protein determination the Exton method has been applied.
The pellets resuspended in distilled water and used for protein determination and toxicity bioassay.
Crude protein determination involved the use of routine Kjeldhal nitrogen assay (N × 6.25).
After that, micro-Kjeldahl procedure [22] was applied for protein determination.
For protein determination, leaves were homogenized in a 50 mM sodium phosphate buffer (pH 6.8).
Following incubation the flow-through (under gravity) was collected (~200μL) and stored at −80°C for protein determination and analysis.
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