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These modifications or changes greatly increased the rigidity of a protein configuration, the property associated with its enhanced thermostability.
The melting temperature (T m) was defined as a temperature, at which a protein configuration is half unfolded.
The emission intensity of the fluorescence dye combining with protein hydrophobic regions increased as the protein configuration was unfolded at high temperature (Niesen et al. 2007).
When Cyt c was encapsulated in ZIF-8, the protecting effect of the framework resisted the protein configuration change in denaturing organic solvents, which resulted in almost unchanged of activity (Fig. 4f).
The mechanism by which LCFA induces fad expression lies in the fact that the binding of LCFA acyl-CoA to FadR protein results in the alteration of protein configuration, which in turn triggers the loss of its DNA binding ability.
The target-specific cellular assay presented here is applicable in high-throughput screens for selecting inhibitors of defined secretases acting on natural substrates in a membrane-bound protein configuration.
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HSPs stabilize target protein configurations and indirectly improve thylakoid membrane structure stability, light energy absorption and passing, electron transport, and fixed carbon assimilation, leading to improved photosynthesis.
The user then selects coordinate files, in PQR format, for all of the protein configurations of interest.
High-resolution structural imaging can be valuable in identifying new protein configurations and protein surface interaction preferences.
The time-evolution of nascent protein configurations is calculated using Brownian dynamics with a 100 ns timestep.
The proposed model evolves a population of protein configurations for a given HP sequence and relies on hill-climbing recombination and mutation to support the search process.
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