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Even though we do not have an explanation for the difference between the in vivo and in vitro observations, this result highlights the need to directly probe protein complex stoichiometry using techniques such as 2dPCH in live-cell settings.
Second, proteins being overproduced at different rates will alter protein complex stoichiometry.
This is the first H-NOX/HaCE protein complex stoichiometry to have been determined.
Such conditions could include metabolic restructuring in response to an increased cell size or to aggregating proteins that are accumulating due to altered protein complex stoichiometry.
Analysis of these ions can provide valuable information about protein complex stoichiometry, protein ligand binding, and specific changes to protein or protein complex structure in solution.
Furthermore, PALM provides a direct way of counting molecules in cells [62 64]; a promising feature for the study of protein complex stoichiometry and quantitative description of reaction networks [46].
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The differential protein content in fetal and adult HSPCs indicate distinct metabolic profiles and protein complex stoichiometries.
Protein complex stoichiometries of ChsE4-ChsE5 were confirmed by LC/UV/MS.
Protein complex stoichiometries of ChsH1-ChsH2 and ChsH1-ChsH2N were confirmed by LC/UV/MS.
Duplicated pigmentation genes follow general evolutionary patterns such as the preservation of protein complex stoichiometries and the overrepresentation of developmental genes among retained duplicates.
In conclusion, the comparison of functional categories of pigmentation genes reveals that the retention of FSGD duplicates, being noticeably high, follows more general WGD duplicate retention patterns, that is, preservation of protein complex stoichiometries and overrepresentation of developmental genes among retained duplicates.
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