Sentence examples for protein blocking from inspiring English sources

After blocking with no protein blocking solution (Sangon Biotech), the membranes were incubated with a primary antibody overnight at 4 °C.

Consequently, the cover slips were incubated with protein blocking working liquid at room temperature for 5 min before the CC49 monoclonal antibody (1 100) was added.

The tetrameric arrangement and the negative charge distribution close to the active sites of the enzyme, have suggested the design of complementary multifunctional receptors that might bind to the active region of the protein blocking the approach of the substrate.

Sections were then treated with 3%H2O22 for 10 min to eliminate endogenous peroxidase activity, used 0.1% avidin and 0.01% biotin for 10 min to block endogenous biotin and avidin activity respectively, and treated with protein blocking agent (Immunon™, Shandon, Pittsburgh, PA, USA) at a 1 1 dilution for 10 min to reduce non-specific binding.

Nonspecific protein binding sites were blocked with serum-free protein blocking solution.

Washed slides were incubated in Serum-free protein blocking buffer (DAKO, Carpenteria, CA) for 30 minutes.

Nonspecific binding was blocked by incubation of slides for 10 minutes with a protein-blocking agent (protein-blocking agent, Dako, Carpinteria, CA, USA) before application of the primary antibody.

A PRL-3 related protein-blocking experiment was performed.

Non-specific binding was blocked for 10 min using protein-blocking buffer.

Non-specific binding was blocked with a protein-blocking reagent (Immunotech, Marseille, France) for 5 min.

The secondary antibody used for CD31 staining (peroxidase-conjugated goat anti-rat IgG) was diluted 1 : 200 in protein-blocking solution.