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§ 520.2380a Thiabendazole top dressing and mineral protein block.
Immunohistochemical staining was performed without protein block prior to incubation with primary Ab.
For immunofluorescence, sections were blocked with serum free protein block (Dako).
Moreover, antibodies against this protein block its biological activity and efficiently protect mice from mousepox, confirming its contribution to virulence22.
Tissue sections were PAP pen applied and preblocked in serum-free protein block for 30 min at ambient temperature.
The endogenous peroxidase activity was blocked with 3% hydrogen peroxide, and sections were additionally blocked with Protein Block Solution (DAKO).
After blocking of nonspecific binding sites with protein block serum free (DAKO), slides were incubated overnight with primary antibodies at 4°C.
For immunohistochemistry as a second staining after ISH, the sections were treated 3% hydrogen peroxide in PBS for 15 min, and Protein Block (Dako).
Serum Free Protein Block (Dako) was used at room temperature for 5 minutes to neutralize any charged molecules on the tissue sections that may cause non specific staining.
Blocking was done with DAKO protein block serum-free for 30 min at RT after washing with phosphate buffered saline (PBS).
Endogenous peroxidase activity was blocked in 3% hydrogen peroxide in phosphate buffered saline (PBS), and non-specific protein binding was blocked with Protein Block (Dako).
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