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From the results reported for Burkina Faso (Lamien et al. 1996), the pulp provides protein amounting to 1.9 g per 100 g of matter and about 4.8 Kcal per 100 g, while the kernel provides protein of 6.8 g per 100 g of matter and 579 Kcal per 100 g.
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Protein amount was measured by BCA assay (Thermo Fisher Scientific).
The same amount of protein was loaded as input to ensure equivalent protein amounts in every reaction.
After recovering the soluble fraction, total protein amount was measured by Pierce TM BCA Protein Assay Kit (ThermoFisher).
The protein amount was the same in all groups, at 20percentt.
Protein amount was quantified by using ImageJ software: intensity of the Hsp90 band was normalized to the total protein amount, i.e. the intensity of the entire lane of the Coomassie-stained gel, run simultaneously (described above).
The membrane after the cleaning remained intact and subsequent filtration left trace protein amount on the membrane surface.
Ishihama, Y. et al. Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein.
= [(Total protein amount − Free protein amount)/Total protein amount] × 100%.
Thus, the protein amount entering the ER is reduced.
Equal protein amounts loading were determined by IgG.
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